Adenoviral or AAV Genomes
Distributed through Addgene, unless otherwise noted.
Link to Pu lab plasmids at Addgene.

Ad:Msln-Cre
Human mesothelin promoter driving Cre. Active in mesothelium.
  • Zhou, B., Honor, L.B., He, H., Ma, Q., Oh, J.H., Butterfield, C., Lin, R.Z., Melero-Martin, J.M., Dolmatova, E., Duffy, H.S., Gise, A., Zhou, P., Hu, Y.W., Wang, G., Zhang, B., Wang, L., Hall, J.L., Moses, M.A., McGowan, F.X., and Pu, W.T. 2011. Adult mouse epicardium modulates myocardial injury by secreting paracrine factors. J Clin Invest. 121:1894-1904.

Ad:TRE-gateway
Tet-activator dependent promoter driving a gateway cassette.
  • He, A. and Pu, W. T. (2010) Curr Protoc Mol Biol Chapter 21, Unit 21.20
  • He, A., Kong, S.W., Ma, Q., and Pu, W.T. 2011. Co-occupancy by multiple cardiac transcription factors identifies transcriptional enhancers active in heart. Proc Natl Acad Sci U S A. 108:5632-5637.

Ad:TNT-rtTA-IRES-BirA
Rat TNT promoter driving the reverse tet activator and BirA, the E. coli enzyme that biotinylates the bio peptide.
  • He, A. and Pu, W. T. (2010) Curr Protoc Mol Biol Chapter 21, Unit 21.20
  • He, A., Kong, S.W., Ma, Q., and Pu, W.T. 2011. Co-occupancy by multiple cardiac transcription factors identifies transcriptional enhancers active in heart. Proc Natl Acad Sci U S A. 108:5632-5637.

Ad:CMV-rtTA-IRES-BirA
CMV promoter driving the reverse get activator and BirA, the E. coli enzyme that biotinylates the bio peptide.
  • He, A. and Pu, W. T. (2010) Curr Protoc Mol Biol Chapter 21, Unit 21.20

  • AAV-cTNT-3Flag-hYAP-S127A
  • Express activated, FLAG-tagged YAP from cardiac specific promoter
  • Lin, Z., von Gise, A., Zhou, P., Gu, F., Ma, Q., Jiang, J., Yau, A. L., Buck, J. N., Gouin, K. A., van Gorp, P. R. R., Zhou, B., Chen, J., Seidman, J. G., Wang, D.-Z. & Pu, W. T. Cardiac-specific YAP activation improves cardiac function and survival in an experimental murine MI model. Circ. Res. 115, 354–363 (2014).

pAAV-cTNT-Luciferase
pAAV.cTNT.iCre
Generate AAV that express Cre or luciferase under control of the cardiac troponin T promoter
  • Prendiville, T. W., Guo, H., Lin, Z., Zhou, P., Stevens, S. M., He, A., VanDusen, N., Chen, J., Zhong, L., Wang, D. Z., Gao, G. & Pu, W. T. Novel Roles of GATA4/6 in the Postnatal Heart Identified through Temporally Controlled, Cardiomyocyte-Specific Gene Inactivation by Adeno-Associated Virus Delivery of Cre Recombinase. PLoS One 10, e0128105 (2015).

AAV-U6gRNA1-U6gRNA2-TNT-Cre
AAV-U6-gRNA-TNT-Cre

Somatic knockout of genes targeted by two guide RNAs in Cre-activated Cas9 mice.
  • Guo, Y., VanDusen, N. J., Zhang, L., Gu, W., Sethi, I., Guatimosim, S., Ma, Q., Jardin, B. D., Ai, Y., Zhang, D., Chen, B., Guo, A., Yuan, G.-C., Song, L.-S. & Pu, W. T. Analysis of Cardiac Myocyte Maturation Using CASAAV, a Platform for Rapid Dissection of Cardiac Myocyte Gene Function In Vivo. Circ. Res. 120, 1874–1888 (2017).
  • VanDusen, N. J., Guo, Y., Gu, W. & Pu, W. T. CASAAV: A CRISPR-Based Platform for Rapid Dissection of Gene Function In Vivo. Curr. Protoc. Mol. Biol. 120, 31.11.1–31.11.14 (2017).

pAAV-CAG-hTAZ
Generate AAV that express human Tafazzin under control of the CAG promoter
  • Wang, S., Li, Y., Xu, Y., Ma, Q., Lin, Z., Schlame, M., Bezzerides, V. J., Strathdee, D. & Pu, W. T. AAV Gene Therapy Prevents and Reverses Heart Failure in A Murine Knockout Model of Barth Syndrome. Circ. Res. (2020). doi:10.1161/CIRCRESAHA.119.315956. Article.