LOCUS       Exported               12527 bp ds-DNA     circular SYN 24-MAR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    PB-TRE-Cas9-EF1a-TetON-T2A-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 24, 2017 from SnapGene Viewer 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12527
                     /organism="synthetic DNA construct"
                     /note="dnas_title=PB-TRE-Cas9-EF1a-TetON-T2A-Puro"
     misc_feature    870..1111
                     /note="3' TF long"
     misc_feature    874..936
                     /note="3' TF short"
     misc_feature    1113..1344
                     /note="Core insulator"
     misc_feature    1298..1317
                     /label=PCR_primer
                     /note="PCR_primer"
                     /note="Insulator fwd seq"
     misc_feature    1339..1388
                     /label=PCR_primer
                     /note="PCR_primer"
                     /note="PB-TRE3G fwd
                     """
     promoter        1364..1739
                     /label=TRE3G
                     /note="TRE3G promoter"
     misc_feature    1612..1631
                     /label=PCR_primer
                     /note="PCR_primer"
                     /note="XR247"
     misc_feature    1723..1743
                     /label=PCR_primer
                     /note="PCR_primer"
                     /note="TRE3G fwd seq"
     misc_feature    1740..1765
                     /note="homology arm"
     gene            1766..5911
                     /note="Cas9"
     misc_feature    2286..2305
                     /note=""
     misc_feature    2794..2815
                     /note=""
     misc_feature    3296..3317
                     /note=""
     misc_feature    3828..3847
                     /note=""
     misc_feature    4361..4381
                     /note=""
     misc_feature    4851..4870
                     /note=""
     misc_feature    5400..5421
                     /note=""
     misc_feature    5952..6223
                     /note="TY polyA"
     misc_feature    6245..6666
                     /note=""
     misc_feature    6245..6273
                     /note=""
     misc_feature    complement(6245..6261)
                     /note=""
     misc_feature    6564..6583
                     /note=""
     misc_feature    complement(6644..6666)
                     /note=""
     misc_feature    6667..7088
                     /note=""
     misc_feature    6667..6695
                     /note=""
     misc_feature    complement(6667..6683)
                     /note=""
     misc_feature    complement(7066..7088)
                     /note=""
     misc_feature    complement(7090..7111)
                     /label=PCR_primer
                     /note="PCR_primer"
                     /note="PB09 seq rvs"
     promoter        7102..7645
                     /note="EF1a promoter"
     misc_feature    complement(7189..7208)
                     /label=PCR_primer
                     /note="PCR_primer"
                     /note="XR248"
     misc_feature    complement(7568..7589)
                     /label=PCR_primer
                     /note="PCR_primer"
                     /note="Ef1a rvs seq"
     misc_feature    7568..7589
                     /label=PCR_primer
                     /note="PCR_primer"
                     /note="(PB_ Ef1a fwd seq)"
     misc_feature    7626..7685
                     /label=PCR_primer
                     /note="PCR_primer"
                     /note="Ef1a-TetON fwd
                     """
     misc_feature    complement(7626..7685)
                     /label=PCR_primer
                     /note="PCR_primer"
                     /note="Ef1a-TetON rvs
                     """
     gene            7658..8401
                     /label=Tet-On 3G
                     /note="Tet-On 3G"
     misc_feature    complement(8377..8426)
                     /label=PCR_primer
                     /note="PCR_primer"
                     /note="TetON-T2A rvs
                     """
     misc_feature    8377..8426
                     /label=PCR_primer
                     /note="PCR_primer"
                     /note="TetON-T2A fwd
                     """
     misc_feature    8402..8455
                     /note="T2A"
     misc_feature    8462..9058
                     /note="Puro"
     misc_feature    complement(9072..9121)
                     /label=PCR_primer
                     /note="PCR_primer"
                     /note="Puro rvs"
     misc_feature    9100..9230
                     /note="SV40 Poly(A)"
     misc_feature    complement(9180..9201)
                     /label=PCR_primer
                     /note="PCR_primer"
                     /note="Rvs primer"
     misc_feature    9478..9708
                     /note="Core insulator"
     misc_feature    9709..10021
                     /note="5' TR long"
     misc_feature    9983..10017
                     /note="5' TR"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     1081 aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca
     1141 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     1201 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     1261 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     1321 acacctgggg ggatacgggg aaaaggcctc cacggccact agttttactc cctatcagtg
     1381 atagagaacg tatgaagagt ttactcccta tcagtgatag agaacgtatg cagactttac
     1441 tccctatcag tgatagagaa cgtataagga gtttactccc tatcagtgat agagaacgta
     1501 tgaccagttt actccctatc agtgatagag aacgtatcta cagtttactc cctatcagtg
     1561 atagagaacg tatatccagt ttactcccta tcagtgatag agaacgtata agctttaggc
     1621 gtgtacggtg ggcgcctata aaagcagagc tcgtttagtg aaccgtcaga tcgcctggag
     1681 caattccaca acacttttgt cttataccaa ctttccgtac cacttcctac cctcgtaaag
     1741 gtctagagct agcgaattcg aatttgccac catggacaag aagtactcca ttgggctcga
     1801 tatcggcaca aacagcgtcg gctgggccgt cattacggac gagtacaagg tgccgagcaa
     1861 aaaattcaaa gttctgggca ataccgatcg ccacagcata aagaagaacc tcattggcgc
     1921 cctcctgttc gactccgggg agacggccga agccacgcgg ctcaaaagaa cagcacggcg
     1981 cagatatacc cgcagaaaga atcggatctg ctacctgcag gagatcttta gtaatgagat
     2041 ggctaaggtg gatgactctt tcttccatag gctggaggag tcctttttgg tggaggagga
     2101 taaaaagcac gagcgccacc caatctttgg caatatcgtg gacgaggtgg cgtaccatga
     2161 aaagtaccca accatatatc atctgaggaa gaagcttgta gacagtactg ataaggctga
     2221 cttgcggttg atctatctcg cgctggcgca tatgatcaaa tttcggggac acttcctcat
     2281 cgagggggac ctgaacccag acaacagcga tgtcgacaaa ctctttatcc aactggttca
     2341 gacttacaat cagcttttcg aagagaaccc gatcaacgca tccggagttg acgccaaagc
     2401 aatcctgagc gctaggctgt ccaaatcccg gcggctcgaa aacctcatcg cacagctccc
     2461 tggggagaag aagaacggcc tgtttggtaa tcttatcgcc ctgtcactcg ggctgacccc
     2521 caactttaaa tctaacttcg acctggccga agatgccaag cttcaactga gcaaagacac
     2581 ctacgatgat gatctcgaca atctgctggc ccagatcggc gaccagtacg cagacctttt
     2641 tttggcggca aagaacctgt cagacgccat tctgctgagt gatattctgc gagtgaacac
     2701 ggagatcacc aaagctccgc tgagcgctag tatgatcaag cgctatgatg agcaccacca
     2761 agacttgact ttgctgaagg cccttgtcag acagcaactg cctgagaagt acaaggaaat
     2821 tttcttcgat cagtctaaaa atggctacgc cggatacatt gacggcggag caagccagga
     2881 ggaattttac aaatttatta agcccatctt ggaaaaaatg gacggcaccg aggagctgct
     2941 ggtaaagctt aacagagaag atctgttgcg caaacagcgc actttcgaca atggaagcat
     3001 cccccaccag attcacctgg gcgaactgca cgctatcctc aggcggcaag aggatttcta
     3061 cccctttttg aaagataaca gggaaaagat tgagaaaatc ctcacatttc ggatacccta
     3121 ctatgtaggc cccctcgccc ggggaaattc cagattcgcg tggatgactc gcaaatcaga
     3181 agagaccatc actccctgga acttcgagga agtcgtggat aagggggcct ctgcccagtc
     3241 cttcatcgaa aggatgacta actttgataa aaatctgcct aacgaaaagg tgcttcctaa
     3301 acactctctg ctgtacgagt acttcacagt ttataacgag ctcaccaagg tcaaatacgt
     3361 cacagaaggg atgagaaagc cagcattcct gtctggagag cagaagaaag ctatcgtgga
     3421 cctcctcttc aagacgaacc ggaaagttac cgtgaaacag ctcaaagaag actatttcaa
     3481 aaagattgaa tgtttcgact ctgttgaaat cagcggagtg gaggatcgct tcaacgcatc
     3541 cctgggaacg tatcacgatc tcctgaaaat cattaaagac aaggacttcc tggacaatga
     3601 ggagaacgag gacattcttg aggacattgt cctcaccctt acgttgtttg aagataggga
     3661 gatgattgaa gaacgcttga aaacttacgc tcatctcttc gacgacaaag tcatgaaaca
     3721 gctcaagagg cgccgatata caggatgggg gcggctgtca agaaaactga tcaatgggat
     3781 ccgagacaag cagagtggaa agacaatcct ggattttctt aagtccgatg gatttgccaa
     3841 ccggaacttc atgcagttga tccatgatga ctctctcacc tttaaggagg acatccagaa
     3901 agcacaagtt tctggccagg gggacagtct tcacgagcac atcgctaatc ttgcaggtag
     3961 cccagctatc aaaaagggaa tactgcagac cgttaaggtc gtggatgaac tcgtcaaagt
     4021 aatgggaagg cataagcccg agaatatcgt tatcgagatg gcccgagaga accaaactac
     4081 ccagaaggga cagaagaaca gtagggaaag gatgaagagg attgaagagg gtataaaaga
     4141 actggggtcc caaatcctta aggaacaccc agttgaaaac acccagcttc agaatgagaa
     4201 gctctacctg tactacctgc agaacggcag ggacatgtac gtggatcagg aactggacat
     4261 caatcggctc tccgactacg acgtggatca tatcgtgccc cagtcttttc tcaaagatga
     4321 ttctattgat aataaagtgt tgacaagatc cgataaaaat agagggaaga gtgataacgt
     4381 cccctcagaa gaagttgtca agaaaatgaa aaattattgg cggcagctgc tgaacgccaa
     4441 actgatcaca caacggaagt tcgataatct gactaaggct gaacgaggtg gcctgtctga
     4501 gttggataaa gccggcttca tcaaaaggca gcttgttgag acacgccaga tcaccaagca
     4561 cgtggcccaa attctcgatt cacgcatgaa caccaagtac gatgaaaatg acaaactgat
     4621 tcgagaggtg aaagttatta ctctgaagtc taagctggtc tcagatttca gaaaggactt
     4681 tcagttttat aaggtgagag agatcaacaa ttaccaccat gcgcatgatg cctacctgaa
     4741 tgcagtggta ggcactgcac ttatcaaaaa atatcccaag cttgaatctg aatttgttta
     4801 cggagactat aaagtgtacg atgttaggaa aatgatcgca aagtctgagc aggaaatagg
     4861 caaggccacc gctaagtact tcttttacag caatattatg aattttttca agaccgagat
     4921 tacactggcc aatggagaga ttcggaagcg accacttatc gaaacaaacg gagaaacagg
     4981 agaaatcgtg tgggacaagg gtagggattt cgcgacagtc cggaaggtcc tgtccatgcc
     5041 gcaggtgaac atcgttaaaa agaccgaagt acagaccgga ggcttctcca aggaaagtat
     5101 cctcccgaaa aggaacagcg acaagctgat cgcacgcaaa aaagattggg accccaagaa
     5161 atacggcgga ttcgattctc ctacagtcgc ttacagtgta ctggttgtgg ccaaagtgga
     5221 gaaagggaag tctaaaaaac tcaaaagcgt caaggaactg ctgggcatca caatcatgga
     5281 gcgatcaagc ttcgaaaaaa accccatcga ctttctcgag gcgaaaggat ataaagaggt
     5341 caaaaaagac ctcatcatta agcttcccaa gtactctctc tttgagcttg aaaacggccg
     5401 gaaacgaatg ctcgctagtg cgggcgagct gcagaaaggt aacgagctgg cactgccctc
     5461 taaatacgtt aatttcttgt atctggccag ccactatgaa aagctcaaag ggtctcccga
     5521 agataatgag cagaagcagc tgttcgtgga acaacacaaa cactaccttg atgagatcat
     5581 cgagcaaata agcgaattct ccaaaagagt gatcctcgcc gacgctaacc tcgataaggt
     5641 gctttctgct tacaataagc acagggataa gcccatcagg gagcaggcag aaaacattat
     5701 ccacttgttt actctgacca acttgggcgc gcctgcagcc ttcaagtact tcgacaccac
     5761 catagacaga aagcggtaca cctctacaaa ggaggtcctg gacgccacac tgattcatca
     5821 gtcaattacg gggctctatg aaacaagaat cgacctctct cagctcggtg gagacagcag
     5881 ggctgacccc aagaagaaga ggaaggtgtg aaagggttcg atccctaccg gttagtaatg
     5941 agtttaaacg ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
     6001 ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa
     6061 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     6121 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     6181 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cagatctgcg aaatcggatc
     6241 catgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc ggtaccaagg
     6301 tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
     6361 ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata
     6421 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
     6481 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
     6541 tgtggaaagg acgaaacacc gggtcatcca cgtactggag ggttttagag ctagaaatag
     6601 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     6661 tttttgtaca aaaaagcagg ctttaaagga accaattcag tcgactggat ccggtaccaa
     6721 ggtcgggcag gaagagggcc tatttcccat gattccttca tatttgcata tacgatacaa
     6781 ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa
     6841 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
     6901 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
     6961 cttgtggaaa ggacgaaaca ccgggtgacc ctcctccagt acggttttag agctagaaat
     7021 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
     7081 ttttttgctg aatctaagtg gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     7141 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     7201 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     7261 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     7321 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     7381 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     7441 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     7501 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     7561 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     7621 tccaagctgt gaccggcgcc tactctagag ccgccatgtc tagactggac aagagcaaag
     7681 tcataaactc tgctctggaa ttactcaatg gagtcggtat cgaaggcctg acgacaagga
     7741 aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc
     7801 gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc cactcctgcc
     7861 ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca taccgctgtg
     7921 ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac
     7981 agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga
     8041 acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac
     8101 aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac
     8161 ttctgaaaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg
     8221 gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga
     8281 ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg
     8341 accttgatat gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggg
     8401 agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc cctgaattca
     8461 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     8521 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     8581 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     8641 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     8701 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     8761 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     8821 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     8881 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     8941 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     9001 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag
     9061 tcgacaatca acctctggat tacaaaattt gtgggttaac ttgtttattg cagcttataa
     9121 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     9181 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga attgactcaa
     9241 atgatgtcaa ttagtctatc agaagctatc tggtctccct tccgggggac aagacatccc
     9301 tgtttaatat ttaaacagca gtgttcccaa actgggttct tatatccctt gctctggtca
     9361 accaggttgc agggtttcct gtcctcacag gaacgaagtc cctaaagaaa cagtggcagc
     9421 caggtttagc cccggaattg actggattcc ttttttaggg cccattggta tggctttttc
     9481 cccgtatccc cccaggtgtc tgcaggctca aagagcagcg agaagcgttc agaggaaagc
     9541 gatcccgtgc caccttcccc gtgcccgggc tgtccccgca cgctgccggc tcggggatgc
     9601 ggggggagcg ccggaccgga gcggagcccc gggcggctcg ctgctgcccc ctagcggggg
     9661 agggacgtaa ttacatccct gggggctttg ggggggggct gtccctgata tctataacaa
     9721 gaaaatatat atataataag ttatcacgta agtagaacat gaaataacaa tataattatc
     9781 gtatgagtta aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt gactcacgcg
     9841 gtcgttatag ttcaaaatca gtgacactta ccgcattgac aagcacgcct cacgggagct
     9901 ccaagcggcg actgagatgt cctaaatgca cagcgacgga ttcgcgctat ttagaaagag
     9961 agagcaatat ttcaagaatg catgcgtcaa ttttacgcag actatctttc tagggttaat
    10021 ctagctgcat caggatcata tcgtcgggtc ttttttccgg ctcagtcatc gcccaagctg
    10081 gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt tcccgcgagg ttgaagcggc
    10141 atggaaagag tttgccgagg atgactgctg ctgcattgac gttgagcgaa aacgcacgtt
    10201 taccatgatg attcgggaag gtgtggccat gcacgccttt aacggtgaac tgttcgttca
    10261 ggccacctgg gataccagtt cgtcgcggct tttccggaca cagttccgga tggtcagccc
    10321 gaagcgcatc agcaacccga acaataccgg cgacagccgg aactgccgtg ccggtgtgca
    10381 gattaatgac agcggtgcgg cgctgggata ttacgtcagc gaggacgggt atcctggctg
    10441 gatgccgcag aaatggacat ggataccccg tgagttaccc ggcgggcgcg cttggcgtaa
    10501 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    10561 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    10621 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    10681 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    10741 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    10801 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    10861 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    10921 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    10981 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    11041 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    11101 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    11161 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    11221 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    11281 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    11341 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    11401 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    11461 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    11521 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    11581 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    11641 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    11701 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    11761 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    11821 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    11881 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    11941 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    12001 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    12061 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    12121 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    12181 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    12241 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    12301 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    12361 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    12421 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    12481 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcat
//
